SYKAM S 433 Automatic Amino Acid Analyser

Innovative automatic Amino Acid Analyzer S 433 combines the advantages of the classical ion exchange separation method with the modern technique of high performance liquid chromatography.

Product Description

The innovative automatic Amino Acid Analyzer S 433 combines the advantages of the classical ion exchange separation method with the modern technique of high performance liquid chromatography. The
complete package of sophisticated instrumentation, a wide variety of prepacked and tested separation columns, combined with optimized ready-to-use buffer solutions and chemicals, creates the right answer for any routine or research problem in amino acid determination. More than 30 years experience in developing and operating sophisticated amino acid
analyzers results in unmatched performance.



Multistep Separation

Only two or three buffer solutions have to be combined to form the best optimized buffer profile at any part of the separation program. No more compromises by the limitation to four or five buffer changes.

Cooled Reagent Storage

All buffer solutions, as well as the Ninhydrine reagent are stored under inert gas pressure in a refrigerated cabinet to avoid oxidation and air contamination independent of the ambient environment.


Integrated Autosampler

with a capacity of 120 vials in a cooled sample tray. Volumes from 1µl to 100 µl can be injected without any loss of sample. The injected volume is adjusted by a high precision syringe, driven by a stepper motor with a resolution of 17 steps per µl. A programmable wash program
will flush the entire injection system to avoid cross contamination of the sample.


Integrated Vacuum Degasser
avoids the interruption of the buffer pump by air bubbles without the need of bubble traps with varying volumes, causing changes in retention times of the different amino acids.


Separation Column Oven
integrated solid state column oven with fast heating and cooling capability with a temperature range from +20°C to +99°C independent of the ambient temperature. Up to twelve programmable temperature steps can be used
during one separation program.


High-Temperature Reactor
with a programmable temperature range from ambient to 180°C with a coiled capillary for the color reaction of the amino acid-ninhydrine complex. Automatic flushing of the reactor coil with a washing solution after each run prevents the blockage of the capillary.


Integrated Reagent Dosing Pump
for Ninhydrine delivery and flushing of the reaction coil after each run. Programmable flow rate from 0.01 to 2.0 ml/min.


Complete Inert Design
All materials coming into contact with the buffer solutions and reagents are made of inert materials as PEEK, PTFE, PVDF etc. Therefore, there is no need for using special non-corrosive buffer compositions or reagents.
Integrated Dual-Channel Photometer for the amino acid detection at 440 nm and 570 nm wavelengths. Summing option for both channels, for
single channel integration of all amino acids.


Safety Devices

control pump pressures, temperatures and leakages.


Optional Application
– Post column derivatisation with OPA (needs an optional
Fluorescence Detector)
– Carbohydrate determination for reducing sugars with
Cu-bicinchoninate post-column derivatisation (except the
separation column, no additional extras are needed)
– Polyamine determination with Ninhydrine or OPA postcolumn derivatisation.



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