GingisKHAN® (Kgp) Smart Enzymes

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. The reducing agent (2 mM cysteine) is provided together with the enzyme.

GingisKHAN is purified from Porphyromonas gingivalis.

GingisKHAN is available in two product formats:

GingisKHAN Enzyme – lyophilized enzyme and reducing agent

GingisKHAN Fab kit – lyophilized enzyme and reducing agent together with affinity purification resin for easy generation and purification of Fab fragments from human IgG1.