Immobilized GlycINATOR® Smart Enzymes

The GlycINATOR enzyme is immobilized on agarose beads and provided in convenient spin columns. The spin columns are available in three different sizes for deglycosylation of up to 0.5 mg (Microspin), 10 mg (Midispin) or 100 mg (Maxispin) IgG. The smallest columns are available in three different pack sizes, with 2, 5, or 10 columns per pack.

The immobilized enzyme hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc intact on the Fc. IgG is incubated with the agarose beads for 15 min and deglycosylated IgG can easily be collected by a 1 minute centrifugation step.

This version of GlycINATOR deglycosylates IgG under physiological reaction conditions, thus preserving the immunoreactivity. Deglycosylation is performed at room temperature. GlycINATOR deglycosylates all human IgG subclasses and IgG from many different species, including mouse, rat, monkey, sheep, goat, cow, and horse.

Storage and Stability

Immobilized GlycINATOR® is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!

Unit Definition

One spin column contains sufficient GlycINATOR-coupled agarose beads to deglycosylate 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) IgG.

GlycINATOR (EndoS2) is an IgG-specific endoglycosidase that hydrolyzes all glycoforms present at the Fc-glycosylation site. The enzyme acts on the chitobiose core and leaves the core GlcNAc intact.